The this genus are of great economic importance, as

The
genus Trichoderma belongs to order
Hypocreales is a filamentous fungus widely distributed in the soils of most
crops producing areas of Iran. Species of this genus are of great economic
importance, as they serve as a source of degrading enzymes (chitinolytic and
cellulytic), antibiotics, plant growth promoters and most commercial
bio-fungicides (Ozbay and Newman, 2004). Trichoderma viride is considered
as a potential bio-control agents for many plant pathogens as well as Pythium
ultimum (Savazzini et al., 2009, Harman et
al, 2004). Mechanisms primarily have included direct effects upon
target fungi via competition, myco-parasitism, and antibiosis. Since the
production of anti-fungal metabolites (chitinase and cellulase enzymes),
appears to be responsible for the ability of Trichoderma strains
to control the plant pathogenic fungi (such as Pythium spp.), it is
hypothesized that superior bio-control activity might be achieved by increasing
production levels of these metabolites. Thus, the induced mutation by gamma
radiation was used to improve the antifungal metabolites in T. viride  in this study. The optimum irradiation dose
was described in previus studies at 250 Gry (Shahbazi et
al, 2014). The cell wall of Pythium spp. is characterized by
its cellulosic nature, and shown to be composed mainly of carbohydrates
(80-90%) plus hexosamines (1-3 %), lipids (3-8 %) and proteins (4%). All
species showed a remarkable similarity in the composition of the cell walls,
the main difference being in the ratios of the glucans present. Three types of
glucans were detected in the cell walls of the Pythium: a ß-D-(1,4)
-linked glucan (cellulose), alinear ß-D-(1,3) -linked glucan and a linear
ß-D-(1,3)-linked glucan with D-glucose substituents attached by ß-(1,6)
linkages (Sietsma et al 1999).  However, even though the Trichoderma strains
are becoming widely used in commercial agriculture, knowledge of its multiple
modes of action is sketchy at best. Proteomics tools permit holistic
examination of protein and gene expression, but most in vitro and in
vivo researches done in the past are poorly suited to such studies. The
purpose of this article is to describe: 
(i) Indicate the effects of induced mutation via gamma radiation (in 250
Gry) on bio-control potential against P. ultimum, (ii) evaluate that the
enzyme production capacities of T. viride
in in vitro condition on P. ultimum biomass induce the key
cellulases for mycoparasitism in Trichoderma, and (iii) proof the
relationship between best cellulase producer (T. viride mutant- TvM21) isolates and highest antagonistic
potential by proteomics analysis.