Introduction: the details of this image, a photograph was

Introduction: Named after a
Danish bacteriologist in 1882, Hans Christian Gram. This method is very common
when identifying bacteria. What is used to create these stains is crystal
violet. This stain can help generate classifications called gram positive.
There is also gram negative, but this is only when the organism is not stained
by the crystal violet. Gram staining is important because it allows us to see
the cell wall of the organism much better. This method, gram staining consists
of staining the bacteria with crystal violet, using the iodine which helps the
dye stay on better, decolorizer and safranin. Gram staining is a differential
stain and it is different from other stain because it uses more than one stain
to prep the bacteria to be observed under the microscope.

Procedure: In the
microbiology lab, how to properly gram stain was taught. Provided to stain was
Escherichia Coli and Staphylococcus Saprophyticus. Smears were made onto the slides
and once this was done, the slides with the smears were then heat fixed
(process in which a slide is placed over a Bunsen Burner to ensure that the
bacteria stay in place). Slides were placed over a sink and then the crystal
violet was then poured all over the slide for a time of 60 seconds. Followed by
a rinse, iodine was then placed, then the decolorizer and lastly the safranin.
After each step, the slides were rinsed off with regular water. Each sample was
the placed under the microscope at each magnification ending on 100x with
immersion oil.

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Results: E. coli was first used in the gram stain lab when placed under the
microscope, Under the 4x magnification, an image was hard to generate. Moving
on to the 10x magnification, a small dark figured appeared. Unable to grasp the
details of this image, a photograph was made zoomed in and shot to get a more
detailed image. Image looked disfigured on this magnification. Onto the 40x
lens, results were still the same. Lastly, with magnification at its highest,
100x with immersion oil, an image was not seen under the scope. Same went for
the staph