Drug abuse is a growing problem that impacts over 20 million people nationwide. Current methods of rehabilitation have proven ineffective with over a 90% likelihood of relapse. Novel animal models such as C. Elegans are key to identify both drug-induced behavior and potential targets for medication development. Their easily quantified behaviors,neural similarities to humans, and dopamine and cholinergic system–dependent attraction make them effective for this study. This experiment challenged C. Elegans with various doses of nicotine resulting in unique responses for concentration levels ranging from 100 ?M to 1000 ?M nicotine. I was also able to examine olfactory preference alterations based on the odor concentrations of substances such as Benzaldehyde through adaptation from pre-exposure. Thermo-sensory and chemosensory cues resulted in associative learning properties strikingly similar to humans in the C. Elegans. The experiment revealed that C. elegans avoid high concentrations of odorants that are deemed attractive at low concentrations due to AWC and ASH sensory neurons. Data pointed to the underperformance of an insulin signaling pathway when exposed, causing the dose-dependent responses. Continuous substance abuse results in increased dependency. Attraction developed in the pretreated elegans within the span of a few hours. This motivation to acquire agents such as ethanol and nicotine morphs into the eventual addiction seen in humans. The insulin signaling system plays a vital role in the motivated behaviors observed. The transient receptor potential (TRP) channels are necessary for an organism to monitor its environment to locate biologically relevant stimuli. In this experiment, I have explored the role for the insulin signaling system in the locomotor response of elegans to an acute nicotine challenge as well as a role for TRP channels in mediating nicotine-approach behavior in these animals. Both of these lines of research promise a superiorly effective rehabilitation process to be created as well as increased knowledge for treatments and medications.