Microorganisms like bacteria have important
use in producing diverse industrial enzyme. This enzyme particularly the
starch-degrading amylolytic is important in biotechnological uses varying from
food, paper, fermentation, textile and pharmaceuticals. Amylase, an enzyme, is
being produced by submerged fermentation. This enzyme can be obtained from some
fungi, bacteria, and actinomycetes. Bacillus
family is important species among the bacterial sources and enzymes used in
various industries, including bacillus
amyloliquefaciens (Naidu and Saranraj, 2013).
According to Cottral (1995), genus bacillus
involves a vast group of gram-positive, oxygen consuming, spore forming rods
which are abundant in soil and are ordinarily found as laboratory contaminants.
Among these enzymes, bacillus family is one of the most widely used for the
mass production of ?-amylase and proteases
(Hardwood, 1992). Belal et al. (2015) add on to this by stating that genus
bacillus have been universally used for enterprise production of enzyme for
various applications. This family is widely used for thermo stable ?-amylase
production to meet industrial needs.
In the study conducted by Castro et al.
(1993b), states that bacillus
amyloliquefaciens makes extracellular ?-amylase
which make it an amylase hyperproducer, and ?-glucosidase, and was obtained
during the course of screening amylolytic bacteria from soil.
In accordance with previous research (Belal et
al., 2015) it has a power to degenerate starch content completely in solid
state culture. Bacillus amyloliquefaciens
was able to consume three types of sugar which are the glucose (simple sugar),
fructose ( ketonic monosaccharide),
and sucrose(disaccharide sugar) as
revealed by Djelal et al. (2016). In their study on comparing Bacillus amyloliquefaciens, Saccharomyces
cerevisiae and Zygosaccharomyces rouxii, they found that bacillus amyloliquefaciens was fitted
for ethanol production from the syrup of dates. Their results stated that ,
among the three tested species, bacillus
amyloliquefaciens was therefore the most efficient species for ethanol
production with rear complete sugar consumption. They explained that the
highest ethanol production among these three species was observed for bacillus amyloliquefaciens.
Manasa et al. (2015) conducted a study about
bioethanol from orange peel. They used Bacillus
subtilis, which is a gram-positive,
catalase-positive bacterium, found in soil and the gastrointestinal tract of
ruminants and humans. This study concluded that Bioethanol was produced with
the addition of 2% sucrose by Bacillus